Genotyping and primer details


Genotyping and primer details for 444G09 - At1g24190
Line ID 444G09
Locus At1g24190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8568132 of line 444G09 Chr1:8568132
   
Insertion allele amplicon 8474 - AB31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB31: 5'-AAAGAGAGGAATAATGGCTCACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8567474
   
Insertion allele amplicon 8474 - AB32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB32: 5'-CAGAACTGCAATCTTTGGTATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8568523
   
wt amplicon (over InsPos) AB32 - AB31
wt amplicon start Chr1:8567474
wt amplicon end Chr1:8568523
wt amplicon size 1050 bp
Locus specific primer AB31: 5'-AAAGAGAGGAATAATGGCTCACAA-3'
2nd locus specific primer AB32: 5'-CAGAACTGCAATCTTTGGTATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11