Genotyping and primer details


Genotyping and primer details for 444H07 - At3g13672
Line ID 444H07
Locus At3g13672 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4474318 of line 444H07 Chr3:4474318
   
Insertion allele amplicon 8474 - 15du
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15du: 5'-ACCCAATTGGATACTTGCTATGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4474679
   
wt amplicon (over InsPos) 01fg - 15du
wt amplicon start Chr3:4473844
wt amplicon end Chr3:4474679
wt amplicon size 836 bp
Locus specific primer 15du: 5'-ACCCAATTGGATACTTGCTATGAC-3'
2nd locus specific primer 01fg: 5'-GAAGTACAAGCAGAATTTTCGACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11