Genotyping and primer details

Genotyping and primer details for 446A08 - At3g61600
Line ID 446A08
Locus At3g61600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22795806 of line 446A08 Chr3:22795806
Insertion allele amplicon 8474 - NC56
Locus specific primer NC56: 5'-ATCAAACCGAACCAAGTCATAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22795516
wt amplicon (over InsPos) 14cu - NC56
wt amplicon start Chr3:22795516
wt amplicon end Chr3:22796342
wt amplicon size 827 bp
Locus specific primer NC56: 5'-ATCAAACCGAACCAAGTCATAAAC-3'
2nd locus specific primer 14cu: 5'-ACAACTGTAGAACAATCCATTCCC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11