Genotyping and primer details


Genotyping and primer details for 446E04 - At2g26730
Line ID 446E04
Locus At2g26730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11391165 of line 446E04 Chr2:11391165
   
Insertion allele amplicon 8474 - EQ70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ70: 5'-GGTTATCATGCTCCTGAAGTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11390840
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11