Genotyping and primer details


Genotyping and primer details for 451A12 - At1g02110
Line ID 451A12
Locus At1g02110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:395528 of line 451A12 Chr1:395528
   
Insertion allele amplicon 8474 - 66rq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 66rq: 5'-CAGAGAGAGGTGAGTCAACATCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:394758
   
wt amplicon (over InsPos) 49sq - 66rq
wt amplicon start Chr1:394758
wt amplicon end Chr1:395621
wt amplicon size 864 bp
Locus specific primer 66rq: 5'-CAGAGAGAGGTGAGTCAACATCAG-3'
2nd locus specific primer 49sq: 5'-TCTTACCCTTGTATTCAATTTGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11