Genotyping and primer details


Genotyping and primer details for 455F06 - At3g20290
Line ID 455F06
Locus At3g20290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7078369 of line 455F06 Chr3:7078369
   
Insertion allele amplicon 8474 - 17vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17vd: 5'-GTTCTGGGGCATATGTGTAAGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7078944
   
wt amplicon (over InsPos) 76vo - 17vd
wt amplicon start Chr3:7077914
wt amplicon end Chr3:7078944
wt amplicon size 1031 bp
Locus specific primer 17vd: 5'-GTTCTGGGGCATATGTGTAAGATT-3'
2nd locus specific primer 76vo: 5'-AGCCAACGAAACAAGCTATAATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11