Genotyping and primer details


Genotyping and primer details for 456A07 - At3g06150
Line ID 456A07
Locus At3g06150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1862311 of line 456A07 Chr3:1862311
   
Insertion allele amplicon 8474 - 29qi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29qi: 5'-ATGAGTGTCAGATTAGCAATGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1862869
   
wt amplicon (over InsPos) 20tf - 29qi
wt amplicon start Chr3:1861927
wt amplicon end Chr3:1862869
wt amplicon size 943 bp
Locus specific primer 29qi: 5'-ATGAGTGTCAGATTAGCAATGACG-3'
2nd locus specific primer 20tf: 5'-ACAAAGAATCCGTGACTCTTCCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11