Genotyping and primer details


Genotyping and primer details for 456B06 - At3g27960
Line ID 456B06
Locus At3g27960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10380723 of line 456B06 Chr3:10380723
   
Insertion allele amplicon 8474 - NC01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NC01: 5'-TAGAGAAACTGCCATCAAGGAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10380243
   
wt amplicon (over InsPos) 03jb - NC01
wt amplicon start Chr3:10380243
wt amplicon end Chr3:10381091
wt amplicon size 849 bp
Locus specific primer NC01: 5'-TAGAGAAACTGCCATCAAGGAGAT-3'
2nd locus specific primer 03jb: 5'-GGGTGTGGTTACTTATATGATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11