Genotyping and primer details


Genotyping and primer details for 458F09 - At4g32480
Line ID 458F09
Locus At4g32480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15676445 of line 458F09 Chr4:15676445
   
Insertion allele amplicon 8474 - 18vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18vd: 5'-AGCATCGAGTATATCCGAATCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15676127
   
wt amplicon (over InsPos) 75vo - 18vd
wt amplicon start Chr4:15676127
wt amplicon end Chr4:15676704
wt amplicon size 578 bp
Locus specific primer 18vd: 5'-AGCATCGAGTATATCCGAATCTGT-3'
2nd locus specific primer 75vo: 5'-ATACCTGGAGAAGTTGTTCGTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11