Genotyping and primer details


Genotyping and primer details for 459C10 - At5g58950
Line ID 459C10
Locus At5g58950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23802146 of line 459C10 Chr5:23802146
   
Insertion allele amplicon 8474 - 13kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13kn: 5'-CCATTAGACAGTTCTTGTGGCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23801563
   
wt amplicon (over InsPos) 02ls - 13kn
wt amplicon start Chr5:23801563
wt amplicon end Chr5:23802586
wt amplicon size 1024 bp
Locus specific primer 13kn: 5'-CCATTAGACAGTTCTTGTGGCATA-3'
2nd locus specific primer 02ls: 5'-ACAAGAGATCATTTAACTTGCGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11