Genotyping and primer details


Genotyping and primer details for 461C12 - At5g50020
Line ID 461C12
Locus At5g50020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20352357 of line 461C12 Chr5:20352357
   
Insertion allele amplicon 8474 - 14kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14kn: 5'-ACATTGGTGAGCTTTATTGGATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20351878
   
wt amplicon (over InsPos) 11le - 14kn
wt amplicon start Chr5:20351878
wt amplicon end Chr5:20352770
wt amplicon size 893 bp
Locus specific primer 14kn: 5'-ACATTGGTGAGCTTTATTGGATTC-3'
2nd locus specific primer 11le: 5'-ATAATCCAAAAGCCACACAAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11