Genotyping and primer details


Genotyping and primer details for 469F11 - At1g70500
Line ID 469F11
Locus At1g70500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26567803 of line 469F11 Chr1:26567803
   
Insertion allele amplicon 8474 - 13di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13di: 5'-TCTGCACCAAACTCTGTTTTATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26568328
   
wt amplicon (over InsPos) 11uo - 13di
wt amplicon start Chr1:26567545
wt amplicon end Chr1:26568328
wt amplicon size 784 bp
Locus specific primer 13di: 5'-TCTGCACCAAACTCTGTTTTATTG-3'
2nd locus specific primer 11uo: 5'-TGTGCTGATTTTACTGTCTTGGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11