Genotyping and primer details


Genotyping and primer details for 470B09 - At2g14520
Line ID 470B09
Locus At2g14520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6183226 of line 470B09 Chr2:6183226
   
Insertion allele amplicon 8474 - 31kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31kh: 5'-GGTAAAGGTGGAGAATTGACACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6183531
   
wt amplicon (over InsPos) 02le - 31kh
wt amplicon start Chr2:6182857
wt amplicon end Chr2:6183531
wt amplicon size 675 bp
Locus specific primer 31kh: 5'-GGTAAAGGTGGAGAATTGACACAT-3'
2nd locus specific primer 02le: 5'-CATTACTCTGTAACGGGTGGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11