Genotyping and primer details


Genotyping and primer details for 471D08 - At3g55410
Line ID 471D08
Locus At3g55410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20544820 of line 471D08 Chr3:20544820
   
Insertion allele amplicon 8474 - 10ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ie: 5'-ATTTATACCTTTCCGGAGCAAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20545211
   
wt amplicon (over InsPos) 09jm - 10ie
wt amplicon start Chr3:20544356
wt amplicon end Chr3:20545211
wt amplicon size 856 bp
Locus specific primer 10ie: 5'-ATTTATACCTTTCCGGAGCAAAGT-3'
2nd locus specific primer 09jm: 5'-CGAGTTTGTTACTTGCATACCAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11