Genotyping and primer details


Genotyping and primer details for 472F03 - At3g09520
Line ID 472F03
Locus At3g09520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2924345 of line 472F03 Chr3:2924345
   
Insertion allele amplicon 8474 - 08bd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08bd: 5'-AAACGAGAAGATAGACTCAATCGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2924714
   
Insertion allele amplicon 8474 - 31aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31aq: 5'-GTGAGGATCCAAACTCTGAGAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2924020
   
wt amplicon (over InsPos) 08bd - 31aq
wt amplicon start Chr3:2924020
wt amplicon end Chr3:2924714
wt amplicon size 695 bp
Locus specific primer 31aq: 5'-GTGAGGATCCAAACTCTGAGAGAC-3'
2nd locus specific primer 08bd: 5'-AAACGAGAAGATAGACTCAATCGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11