Genotyping and primer details


Genotyping and primer details for 472H07 - At5g04540
Line ID 472H07
Locus At5g04540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1298145 of line 472H07 Chr5:1298145
   
Insertion allele amplicon 8474 - 26qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26qz: 5'-TGCATAAGCAGCCCTTATAAGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1298498
   
wt amplicon (over InsPos) 02rp - 26qz
wt amplicon start Chr5:1297582
wt amplicon end Chr5:1298498
wt amplicon size 917 bp
Locus specific primer 26qz: 5'-TGCATAAGCAGCCCTTATAAGATT-3'
2nd locus specific primer 02rp: 5'-TAAAATGGGTTACAGTGGACATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11