Genotyping and primer details


Genotyping and primer details for 474A08 - At5g58850
Line ID 474A08
Locus At5g58850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23764326 of line 474A08 Chr5:23764326
   
Insertion allele amplicon 8474 - 07md
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07md: 5'-GAATTTAATGGGTCATTCATTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23763800
   
wt amplicon (over InsPos) 09mx - 07md
wt amplicon start Chr5:23763800
wt amplicon end Chr5:23764909
wt amplicon size 1110 bp
Locus specific primer 07md: 5'-GAATTTAATGGGTCATTCATTGGT-3'
2nd locus specific primer 09mx: 5'-TTCTTCTCGTCATTGTCCTTGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11