Genotyping and primer details


Genotyping and primer details for 475G11 - At3g13450
Line ID 475G11
Locus At3g13450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4383976 of line 475G11 Chr3:4383976
   
Insertion allele amplicon 8474 - 08ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ht: 5'-TCAAATTTGTGTGATCTTCTCCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4384400
   
wt amplicon (over InsPos) 04io - 08ht
wt amplicon start Chr3:4383541
wt amplicon end Chr3:4384400
wt amplicon size 860 bp
Locus specific primer 08ht: 5'-TCAAATTTGTGTGATCTTCTCCAG-3'
2nd locus specific primer 04io: 5'-TGTCACATAAACCCCAATGAGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11