Genotyping and primer details


Genotyping and primer details for 482A05 - At1g15800
Line ID 482A05
Locus At1g15800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5442394 of line 482A05 Chr1:5442394
   
Insertion allele amplicon 8474 - 06ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ve: 5'-TCCTATTTTTGCAATCTAGTGCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5442827
   
wt amplicon (over InsPos) 79vs - 06ve
wt amplicon start Chr1:5441764
wt amplicon end Chr1:5442827
wt amplicon size 1064 bp
Locus specific primer 06ve: 5'-TCCTATTTTTGCAATCTAGTGCCT-3'
2nd locus specific primer 79vs: 5'-CATTCCTTTCCTTTAAGAGCACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11