Genotyping and primer details


Genotyping and primer details for 485C03 - At5g60300
Line ID 485C03
Locus At5g60300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24266584 of line 485C03 Chr5:24266584
   
Insertion allele amplicon 8474 - EQ79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ79: 5'-GGTATTGCAGAAGAAAACGAGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24266075
   
Insertion allele amplicon 8474 - KU54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU54: 5'-CTTCTCTGGTTCTGCAACTATGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24266937
   
wt amplicon (over InsPos) KU54 - EQ79
wt amplicon start Chr5:24266075
wt amplicon end Chr5:24266937
wt amplicon size 863 bp
Locus specific primer EQ79: 5'-GGTATTGCAGAAGAAAACGAGAAC-3'
2nd locus specific primer KU54: 5'-CTTCTCTGGTTCTGCAACTATGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11