Genotyping and primer details


Genotyping and primer details for 493C09 - At3g53670
Line ID 493C09
Locus At3g53670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19891372 of line 493C09 Chr3:19891372
   
Insertion allele amplicon 8474 - 03bh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03bh: 5'-CTAGCTCGGAGTAAGCTAATCGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19890920
   
wt amplicon (over InsPos) 60dy - 03bh
wt amplicon start Chr3:19890920
wt amplicon end Chr3:19891915
wt amplicon size 996 bp
Locus specific primer 03bh: 5'-CTAGCTCGGAGTAAGCTAATCGAA-3'
2nd locus specific primer 60dy: 5'-CAGGAATCAGAGTAACCACACTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11