Genotyping and primer details


Genotyping and primer details for 494A04 - At4g28250
Line ID 494A04
Locus At4g28250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14000950 of line 494A04 Chr4:14000950
   
Insertion allele amplicon 8474 - JV23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JV23: 5'-CGTGGACTAATCCCAGTCATTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14001417
   
wt amplicon (over InsPos) 02nv - JV23
wt amplicon start Chr4:14000611
wt amplicon end Chr4:14001417
wt amplicon size 807 bp
Locus specific primer JV23: 5'-CGTGGACTAATCCCAGTCATTTAT-3'
2nd locus specific primer 02nv: 5'-CCATACATGCTTCATCTCTAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11