Genotyping and primer details


Genotyping and primer details for 494B07 - At4g29770
Line ID 494B07
Locus At4g29770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14576793 of line 494B07 Chr4:14576793
   
Insertion allele amplicon 8474 - GA74
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GA74: 5'-TTTTCCTCTGCAACTAGAAATGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14576175
   
wt amplicon (over InsPos) 03ks - GA74
wt amplicon start Chr4:14576175
wt amplicon end Chr4:14577253
wt amplicon size 1079 bp
Locus specific primer GA74: 5'-TTTTCCTCTGCAACTAGAAATGGT-3'
2nd locus specific primer 03ks: 5'-CATATCGTGTTTCATTACCTGACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11