Genotyping and primer details


Genotyping and primer details for 495F04 - At1g02610
Line ID 495F04
Locus At1g02610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:554108 of line 495F04 Chr1:554108
   
Insertion allele amplicon 8474 - 17nm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17nm: 5'-AAAAAGAAGAGCAATTTACCGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:553581
   
wt amplicon (over InsPos) 16vu - 17nm
wt amplicon start Chr1:553581
wt amplicon end Chr1:554813
wt amplicon size 1233 bp
Locus specific primer 17nm: 5'-AAAAAGAAGAGCAATTTACCGTGA-3'
2nd locus specific primer 16vu: 5'-AACAGCAGTCACAATCAGGTAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11