Genotyping and primer details


Genotyping and primer details for 499H12 - At2g46160
Line ID 499H12
Locus At2g46160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18963415 of line 499H12 Chr2:18963415
   
Insertion allele amplicon 8474 - CC70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC70: 5'-CGTATTTTATTTTGAACCGCTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18964007
   
Insertion allele amplicon 8474 - CC71
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC71: 5'-TAAACAGTCAACGTTACATGGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18962793
   
wt amplicon (over InsPos) 52vh - CC71
wt amplicon start Chr2:18962793
wt amplicon end Chr2:18963883
wt amplicon size 1090 bp
Locus specific primer CC71: unknown
2nd locus specific primer 52vh: 5'-AATTAATGGCAAACTCCACTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11