Genotyping and primer details


Genotyping and primer details for 502H09 - At5g24650
Line ID 502H09
Locus At5g24650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8437720 of line 502H09 Chr5:8437720
   
Insertion allele amplicon 8474 - 13ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ie: 5'-GAAAAACACTCCTTGAAAAACAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8438310
   
wt amplicon (over InsPos) 01jf - 13ie
wt amplicon start Chr5:8437341
wt amplicon end Chr5:8438310
wt amplicon size 970 bp
Locus specific primer 13ie: 5'-GAAAAACACTCCTTGAAAAACAGC-3'
2nd locus specific primer 01jf: 5'-AACTTTATCTCCTGAAATGCCTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11