Genotyping and primer details


Genotyping and primer details for 503A04 - At4g19110
Line ID 503A04
Locus At4g19110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10457388 of line 503A04 Chr4:10457388
   
Insertion allele amplicon 8474 - GA60
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GA60: 5'-ATATCTTGTTCTTATGGGTGCTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10457874
   
wt amplicon (over InsPos) 01nl - GA60
wt amplicon start Chr4:10456980
wt amplicon end Chr4:10457874
wt amplicon size 895 bp
Locus specific primer GA60: 5'-ATATCTTGTTCTTATGGGTGCTGG-3'
2nd locus specific primer 01nl: 5'-ATATCATTTTCCCGGATTACTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11