Genotyping and primer details


Genotyping and primer details for 510G08 - At1g60630
Line ID 510G08
Locus At1g60630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22335082 of line 510G08 Chr1:22335082
   
Insertion allele amplicon 8474 - 01se
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01se: 5'-ATTGCACACACACTCTTCTCTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22335470
   
Insertion allele amplicon 8474 - EQ84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ84: 5'-ACTCAGTTTTGTTAAGCAACCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22334621
   
wt amplicon (over InsPos) 01se - EQ84
wt amplicon start Chr1:22334621
wt amplicon end Chr1:22335470
wt amplicon size 850 bp
Locus specific primer EQ84: 5'-ACTCAGTTTTGTTAAGCAACCCAT-3'
2nd locus specific primer 01se: 5'-ATTGCACACACACTCTTCTCTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11