Genotyping and primer details


Genotyping and primer details for 512E05 - At5g10160
Line ID 512E05
Locus At5g10160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3186185 of line 512E05 Chr5:3186185
   
Insertion allele amplicon 8474 - 63ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 63ra: 5'-CTCTTCATCAGTCCCTATCTCCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3185877
   
wt amplicon (over InsPos) 29rt - 63ra
wt amplicon start Chr5:3185877
wt amplicon end Chr5:3186585
wt amplicon size 709 bp
Locus specific primer 63ra: 5'-CTCTTCATCAGTCCCTATCTCCAC-3'
2nd locus specific primer 29rt: 5'-AAAACTTGAACACAAATTTCACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11