Genotyping and primer details


Genotyping and primer details for 517E09 - At3g03550
Line ID 517E09
Locus At3g03550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:851296 of line 517E09 Chr3:851296
   
Insertion allele amplicon 8474 - 21pq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21pq: 5'-GTATTAACACCATCATGCAAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:851659
   
wt amplicon (over InsPos) 12ua - 21pq
wt amplicon start Chr3:850892
wt amplicon end Chr3:851659
wt amplicon size 768 bp
Locus specific primer 21pq: 5'-GTATTAACACCATCATGCAAAAGC-3'
2nd locus specific primer 12ua: 5'-CCAAGTATCAATACAAGGAACGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11