Genotyping and primer details


Genotyping and primer details for 518H10 - At2g42230
Line ID 518H10
Locus At2g42230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17594478 of line 518H10 Chr2:17594478
   
Insertion allele amplicon 8474 - 28uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28uj: 5'-GAAATTAACCCACGAACACAATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17594157
   
wt amplicon (over InsPos) 08uu - 28uj
wt amplicon start Chr2:17594157
wt amplicon end Chr2:17594855
wt amplicon size 699 bp
Locus specific primer 28uj: 5'-GAAATTAACCCACGAACACAATCT-3'
2nd locus specific primer 08uu: 5'-AGTTGAGCATTGTGAGAGAGTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11