Genotyping and primer details


Genotyping and primer details for 527A02 - At4g29780
Line ID 527A02
Locus At4g29780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14581227 of line 527A02 Chr4:14581227
   
Insertion allele amplicon 8474 - 34kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34kh: 5'-CACTTGTACTTCTGTCTGAGCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14581803
   
wt amplicon (over InsPos) 03ky - 34kh
wt amplicon start Chr4:14580687
wt amplicon end Chr4:14581803
wt amplicon size 1117 bp
Locus specific primer 34kh: 5'-CACTTGTACTTCTGTCTGAGCCTG-3'
2nd locus specific primer 03ky: 5'-TCAACTTGCCACAAACTAGTCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11