Genotyping and primer details


Genotyping and primer details for 528F07 - At3g08870
Line ID 528F07
Locus At3g08870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2702320 of line 528F07 Chr3:2702320
   
Insertion allele amplicon 8474 - 57wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 57wp: 5'-ATTGGACCCGAAGAAGATAGATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2701404
   
Insertion allele amplicon 8474 - EQ90
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ90: 5'-TTTCACTTTCCATAATGGGCTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2702906
   
Insertion allele amplicon 8474 - KR76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR76: 5'-GGTTGTTGAAATTAAGACCGATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2702041
   
wt amplicon (over InsPos) KR76 - EQ90
wt amplicon start Chr3:2702041
wt amplicon end Chr3:2702906
wt amplicon size 866 bp
Locus specific primer EQ90: 5'-TTTCACTTTCCATAATGGGCTAAC-3'
2nd locus specific primer KR76: 5'-GGTTGTTGAAATTAAGACCGATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11