Genotyping and primer details


Genotyping and primer details for 529E07 - At4g26550
Line ID 529E07
Locus At4g26550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13403722 of line 529E07 Chr4:13403722
   
Insertion allele amplicon 8474 - JL71
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL71: 5'-AGGTCCAATATTGTTTTCCACTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13403130
   
wt amplicon (over InsPos) 18gl - JL71
wt amplicon start Chr4:13403130
wt amplicon end Chr4:13404212
wt amplicon size 1083 bp
Locus specific primer JL71: 5'-AGGTCCAATATTGTTTTCCACTTG-3'
2nd locus specific primer 18gl: 5'-AAAAATGTCACAAGGATGGTTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11