Genotyping and primer details


Genotyping and primer details for 529F02 - At3g27560
Line ID 529F02
Locus At3g27560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10212488 of line 529F02 Chr3:10212488
   
Insertion allele amplicon 8474 - 36aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36aq: 5'-ACATTTTCAATCCATCAAACAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10212073
   
wt amplicon (over InsPos) 03ax - 36aq
wt amplicon start Chr3:10212073
wt amplicon end Chr3:10212853
wt amplicon size 781 bp
Locus specific primer 36aq: 5'-ACATTTTCAATCCATCAAACAACA-3'
2nd locus specific primer 03ax: 5'-TCAACTTCAGTTCTTCTTCCCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11