Genotyping and primer details


Genotyping and primer details for 530B04 - At1g31230
Line ID 530B04
Locus At1g31230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11161854 of line 530B04 Chr1:11161854
   
Insertion allele amplicon 8474 - 30cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30cs: 5'-ATCCACTATCAGCTCACCCTTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11161307
   
wt amplicon (over InsPos) 03lz - 30cs
wt amplicon start Chr1:11161307
wt amplicon end Chr1:11162223
wt amplicon size 917 bp
Locus specific primer 30cs: 5'-ATCCACTATCAGCTCACCCTTCTA-3'
2nd locus specific primer 03lz: 5'-AGATGTTAGCTGCTGTTGTGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11