Genotyping and primer details


Genotyping and primer details for 530C12 - At3g56360
Line ID 530C12
Locus At3g56360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20897329 of line 530C12 Chr3:20897329
   
Insertion allele amplicon 8474 - 02bd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02bd: 5'-GGCTTTGCACTATGATCACTAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20897695
   
Insertion allele amplicon 8474 - MN35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN35: 5'-TCTTAGAGAGTCGCCGTTATTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20896928
   
wt amplicon (over InsPos) 02bd - MN35
wt amplicon start Chr3:20896928
wt amplicon end Chr3:20897695
wt amplicon size 768 bp
Locus specific primer MN35: 5'-TCTTAGAGAGTCGCCGTTATTGAT-3'
2nd locus specific primer 02bd: 5'-GGCTTTGCACTATGATCACTAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11