Genotyping and primer details


Genotyping and primer details for 530E08 - At3g01930
Line ID 530E08
Locus At3g01930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:320593 of line 530E08 Chr3:320593
   
Insertion allele amplicon 8474 - 51gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51gp: 5'-TGCAACTGAGCAATTCTCTTATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:320122
   
wt amplicon (over InsPos) 25hs - 51gp
wt amplicon start Chr3:320122
wt amplicon end Chr3:321037
wt amplicon size 916 bp
Locus specific primer 51gp: 5'-TGCAACTGAGCAATTCTCTTATGT-3'
2nd locus specific primer 25hs: 5'-AGTTTGCGTTTTTAAGGTTGTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11