Genotyping and primer details


Genotyping and primer details for 534A11 - At1g77330
Line ID 534A11
Locus At1g77330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29064320 of line 534A11 Chr1:29064320
   
Insertion allele amplicon 8474 - 07gt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07gt: 5'-GAGATTTTTGAAAATGGGTTTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29063981
   
wt amplicon (over InsPos) 03mj - 07gt
wt amplicon start Chr1:29063981
wt amplicon end Chr1:29064914
wt amplicon size 934 bp
Locus specific primer 07gt: 5'-GAGATTTTTGAAAATGGGTTTGAA-3'
2nd locus specific primer 03mj: 5'-TGCCAAGGATTATACAAATGTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11