Genotyping and primer details


Genotyping and primer details for 535C09 - At3g33530
Line ID 535C09
Locus At3g33530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:14093369 of line 535C09 Chr3:14093369
   
Insertion allele amplicon 8474 - 22sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22sf: 5'-CAACACATTTAGAAGGATCGGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:14092846
   
wt amplicon (over InsPos) 02sz - 22sf
wt amplicon start Chr3:14092846
wt amplicon end Chr3:14093808
wt amplicon size 963 bp
Locus specific primer 22sf: 5'-CAACACATTTAGAAGGATCGGATT-3'
2nd locus specific primer 02sz: 5'-AGACGATTCTTTCATTGAGGCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11