Genotyping and primer details


Genotyping and primer details for 537F08 - At4g27650
Line ID 537F08
Locus At4g27650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13804749 of line 537F08 Chr4:13804749
   
Insertion allele amplicon 3144 - CT37
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CT37: 5'-AGCAGAGAATCTCAGGCTGTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13804363
   
Insertion allele amplicon 8474 - NC52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NC52: 5'-AAAAAGCGGAAAGAGTTTCACATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13805089
   
wt amplicon (over InsPos) NC52 - CT37
wt amplicon start Chr4:13804363
wt amplicon end Chr4:13805089
wt amplicon size 727 bp
Locus specific primer CT37: 5'-AGCAGAGAATCTCAGGCTGTTTAG-3'
2nd locus specific primer NC52: 5'-AAAAAGCGGAAAGAGTTTCACATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11