Genotyping and primer details


Genotyping and primer details for 540H12 - At5g49460
Line ID 540H12
Locus At5g49460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20057621 of line 540H12 Chr5:20057621
   
Insertion allele amplicon 8474 - KR53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR53: 5'-AACAAACAGACCGTTGAGATAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20058093
   
wt amplicon (over InsPos) 15em - KR53
wt amplicon start Chr5:20057156
wt amplicon end Chr5:20058093
wt amplicon size 938 bp
Locus specific primer KR53: 5'-AACAAACAGACCGTTGAGATAACC-3'
2nd locus specific primer 15em: 5'-GTCATTTCCCTTCTATGGTTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11