Genotyping and primer details


Genotyping and primer details for 541A03 - At3g60600
Line ID 541A03
Locus At3g60600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22401869 of line 541A03 Chr3:22401869
   
Insertion allele amplicon 8474 - 49fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49fr: 5'-CATGGTTCTGAATTTTCGGTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22401423
   
wt amplicon (over InsPos) 28go - 49fr
wt amplicon start Chr3:22401423
wt amplicon end Chr3:22402415
wt amplicon size 993 bp
Locus specific primer 49fr: 5'-CATGGTTCTGAATTTTCGGTTAGT-3'
2nd locus specific primer 28go: 5'-GGGTGGTTTATGTCCTCTTCATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11