Genotyping and primer details


Genotyping and primer details for 549B06 - At2g32730
Line ID 549B06
Locus At2g32730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13883885 of line 549B06 Chr2:13883885
   
Insertion allele amplicon 8474 - 26et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26et: 5'-TCATTCAACATGGAGCTTGTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13883388
   
wt amplicon (over InsPos) 04fz - 26et
wt amplicon start Chr2:13883388
wt amplicon end Chr2:13884236
wt amplicon size 849 bp
Locus specific primer 26et: 5'-TCATTCAACATGGAGCTTGTTTAG-3'
2nd locus specific primer 04fz: 5'-CTTGACGAACAAAGTCAACAACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11