Genotyping and primer details


Genotyping and primer details for 554E02 - At5g20420
Line ID 554E02
Locus At5g20420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6900910 of line 554E02 Chr5:6900910
   
Insertion allele amplicon 8474 - 25jn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25jn: 5'-ACGAAATGTTATGGAGAGAAATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6901335
   
wt amplicon (over InsPos) 54vh - 25jn
wt amplicon start Chr5:6900548
wt amplicon end Chr5:6901335
wt amplicon size 788 bp
Locus specific primer 25jn: 5'-ACGAAATGTTATGGAGAGAAATGG-3'
2nd locus specific primer 54vh: 5'-AGTTGTTTTAGGAGCAAGAACCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11