Genotyping and primer details


Genotyping and primer details for 556H02 - At3g11490
Line ID 556H02
Locus At3g11490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3618180 of line 556H02 Chr3:3618180
   
Insertion allele amplicon 8474 - 45hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45hw: 5'-ATACTAGAGGGAACATCGTGCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3618655
   
wt amplicon (over InsPos) 19ix - 45hw
wt amplicon start Chr3:3617557
wt amplicon end Chr3:3618655
wt amplicon size 1099 bp
Locus specific primer 45hw: 5'-ATACTAGAGGGAACATCGTGCCTA-3'
2nd locus specific primer 19ix: 5'-TTCACACGTCCCACAATACTACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11