Genotyping and primer details


Genotyping and primer details for 557G05 - At1g17820
Line ID 557G05
Locus At1g17820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6134078 of line 557G05 Chr1:6134078
   
Insertion allele amplicon 8474 - 29vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29vf: 5'-CTGAATTCTTCACTCCTGTTTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6133608
   
wt amplicon (over InsPos) 23vu - 29vf
wt amplicon start Chr1:6133608
wt amplicon end Chr1:6134847
wt amplicon size 1240 bp
Locus specific primer 29vf: 5'-CTGAATTCTTCACTCCTGTTTTCC-3'
2nd locus specific primer 23vu: 5'-TTCTGGGTTTTGTTCCTTCTCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11