Genotyping and primer details


Genotyping and primer details for 557G10 - At3g14075
Line ID 557G10
Locus At3g14075 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4663968 of line 557G10 Chr3:4663968
   
Insertion allele amplicon 8474 - 29uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29uj: 5'-AACAATGTTCAGGTTCATTTGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4663339
   
wt amplicon (over InsPos) 45vw - 29uj
wt amplicon start Chr3:4663339
wt amplicon end Chr3:4664499
wt amplicon size 1161 bp
Locus specific primer 29uj: 5'-AACAATGTTCAGGTTCATTTGACA-3'
2nd locus specific primer 45vw: 5'-AGAGCCTCTGCTACTCAGTCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11