Genotyping and primer details


Genotyping and primer details for 562F09 - At3g04830
Line ID 562F09
Locus At3g04830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1328980 of line 562F09 Chr3:1328980
   
Insertion allele amplicon 8474 - 16kw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16kw: 5'-CACTGGAAGTTCATTGTCATTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1328522
   
wt amplicon (over InsPos) 12ls - 16kw
wt amplicon start Chr3:1328522
wt amplicon end Chr3:1329574
wt amplicon size 1053 bp
Locus specific primer 16kw: 5'-CACTGGAAGTTCATTGTCATTCAT-3'
2nd locus specific primer 12ls: 5'-GTATTTACTTGCATCTAATGGCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11