Genotyping and primer details


Genotyping and primer details for 564D07 - At3g44830
Line ID 564D07
Locus At3g44830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16371845 of line 564D07 Chr3:16371845
   
Insertion allele amplicon 8474 - 17gd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17gd: 5'-CTCAAAACATCTTCAATGAGTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16372262
   
wt amplicon (over InsPos) 01hy - 17gd
wt amplicon start Chr3:16371481
wt amplicon end Chr3:16372262
wt amplicon size 782 bp
Locus specific primer 17gd: 5'-CTCAAAACATCTTCAATGAGTCCA-3'
2nd locus specific primer 01hy: 5'-AGTCCAAAACATAGTCCCATTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11